Mass Spectrometry of Glycans
ثبت نشده
چکیده
The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies. Unlike the HPLC method, a substantially larger amount (~10‐20 times) of glycan is normally required for a single MS spectrum. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is the most widely used MS technique. The information gained by using MALDI MS is mass weight, which can be used to assign putative monosaccharide structures present in a pure oligosaccharide since the mass of a monosaccharide is measured with a high degree of accuracy. MALDI MS can be used in conjunction with glycan sequencing using exoglycosidase enzymes for structural and linkage analysis of glycans. See Glycan Sequencing starting on page 85 for further information.
منابع مشابه
DNA-mediated cell surface engineering for multiplexed glycan profiling using MALDI-TOF mass spectrometry
Glycans are crucial for many key biological processes and their alterations are often a hallmark of disease. Thus, multiplexed and sensitive analysis of glycans is of intense interest. Here we report a novel approach using DNA-mediated cell surface engineering for glycan profiling by MALDI-TOF mass spectrometry (MS). Following lectin binding, DNA amplification and hybridization, glycans on the ...
متن کاملStructure determination by MALDI-IRMPD mass spectrometry and exoglycosidase digestions of O-linked oligosaccharides from Xenopus borealis egg jelly.
Differences in the fertilization behavior of Xenopus borealis from X. laevis and X. tropicalis suggest differences in the glycosylation of the egg jellies. To test this assumption, O-linked glycans were chemically released from the egg jelly coat glycoproteins of X. borealis. Over 50 major neutral glycans were observed, and no anionic glycans were detected from the released O-glycan pool. Preli...
متن کاملImaging of N-Linked Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using MALDI Mass Spectrometry
Aberrant glycosylation is associated with most of the diseases. Direct imaging and profiling of N-glycans on tissue sections can reveal tissue-specific and/or disease-associated N-glycans, which not only could serve as molecular signatures for diagnosis but also shed light on the functional roles of these biomolecules. Mass spectrometry imaging (MSI) is a powerful tool that has been used to cor...
متن کاملTwo-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry
A new mass spectrometry imaging approach to simultaneously map the two-dimensional distribution of N-glycans in tissues has been recently developed. The method uses Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS) to spatially profile the location and distribution of multiple N-linked glycan species released by peptide N-glycosidase F in frozen or formalin-fixed...
متن کاملSite Specific Identification of N-linked Glycosylation in Proteins by Liquid Chromatography–electrospray Ionization Tandem Mass Spectrometry
Abstract. Recently, we reported the characterization of the glycans attached at the 11 N-glycosylation sites of Hepatitis C virus E2 envelope glycoprotein by tandem mass spectrometry. Infections caused by Hepatitis C virus represent the main cause of liver diseases such as hepatitis, cirrhosis and hepatocellular carcinoma. The N-linked sugars consist primarily of high mannose glycans, with stru...
متن کاملStructural Identification of Serum N-glycans Associated with Cancer Progression
Microchip electrophoresis is used (i) to assign structures of serum N-glycans and (ii) quantitatively compare samples derived from ovarian cancer patients and disease-free (control) individuals. Neutralization of native charges from sialic acids present on serum N-glycans allowed direct correlation between electrophoretic mobility and glycan mass. From a calibration curve of the masses and mobi...
متن کامل